rabbit anti glur2 3 Search Results


rabbit-anti-glur2/3 (glutamate receptor subunits 2/3  (Merck & Co)
90
Merck & Co rabbit-anti-glur2/3 (glutamate receptor subunits 2/3
The architecture of ribbon synapses appears normal in BACE1−/− mice. A, B, Representative maximum intensity projections of confocal z-stacks from the IHC region in the organ of Corti of the apical turn of (A) wild-type and (B) and BACE1−/− mice stained with antibodies directed against presynaptic CtBP2 (red) and postsynaptic <t>GluR2/3</t> (green). Dotted lines visualize individual IHCs in the region. C, CtBP2 and GluR2/3 puncta were quantified by blinded counting of immunopositive clusters from images as shown in (A+B) in BACE1+/+ (n = 41 cells of 3 mice) and BACE1−/− mice (n = 57 cells of 3 mice). There was no significant difference between genotypes. D, There was no obvious difference in the number of CtBP2-positive clusters in OHCs of BACE1+/+ (left) and BACE1−/− (right) mice. D, Representative confocal projections through the OHC region in the apical turn of BACE1+/+ (left) and BACE1−/− (right) mice stained with anti-CtBP2 antibodies (red) and DAPI (blue).
Rabbit Anti Glur2/3 (Glutamate Receptor Subunits 2/3, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit-anti-glur2/3 (glutamate receptor subunits 2/3/product/Merck & Co
Average 90 stars, based on 1 article reviews
rabbit-anti-glur2/3 (glutamate receptor subunits 2/3 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti-glur2&3 polyclonal antibody  (Fisher Scientific)
90
Fisher Scientific rabbit anti-glur2&3 polyclonal antibody
FGF22 depletion reduces ribbon synapse number in vivo. AAV-shFGF22 and AAV-FGF22 were administrated to mouse cochlea to deplete or overexpress FGF22 in hair cells, respectively. Co-administration of AAV-shMEF2D with AAV-shFGF22 was also performed to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. Similarly, co-administration of AAV-MEF2D with AAV-FGF22 was performed, also to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. ( A , B ) TUNEL staining was performed on cochlea, showing by representative images ( A ), and by quantification ( B ). ( C , D ) The ribbon synapses were determined by co-staining for CtBP2 (in green) and <t>GluR2&3</t> (in red). Cochlear ribbon synapse number was assessed, shown by representative images ( C ), and by quantification ( D ). *p<0.05. NS: non-significant. N=5. Scale bar is 20μm.
Rabbit Anti Glur2&3 Polyclonal Antibody, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-glur2&3 polyclonal antibody/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
rabbit anti-glur2&3 polyclonal antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti-kainate/2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (ampa) glutamate receptor (glur) 2/3  (Cedarlane)
90
Cedarlane rabbit anti-kainate/2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (ampa) glutamate receptor (glur) 2/3
FGF22 depletion reduces ribbon synapse number in vivo. AAV-shFGF22 and AAV-FGF22 were administrated to mouse cochlea to deplete or overexpress FGF22 in hair cells, respectively. Co-administration of AAV-shMEF2D with AAV-shFGF22 was also performed to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. Similarly, co-administration of AAV-MEF2D with AAV-FGF22 was performed, also to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. ( A , B ) TUNEL staining was performed on cochlea, showing by representative images ( A ), and by quantification ( B ). ( C , D ) The ribbon synapses were determined by co-staining for CtBP2 (in green) and <t>GluR2&3</t> (in red). Cochlear ribbon synapse number was assessed, shown by representative images ( C ), and by quantification ( D ). *p<0.05. NS: non-significant. N=5. Scale bar is 20μm.
Rabbit Anti Kainate/2 Amino 3 (3 Hydroxy 5 Methyl Isoxazol 4 Yl)Propanoic Acid (Ampa) Glutamate Receptor (Glur) 2/3, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-kainate/2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (ampa) glutamate receptor (glur) 2/3/product/Cedarlane
Average 90 stars, based on 1 article reviews
rabbit anti-kainate/2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl)propanoic acid (ampa) glutamate receptor (glur) 2/3 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


The architecture of ribbon synapses appears normal in BACE1−/− mice. A, B, Representative maximum intensity projections of confocal z-stacks from the IHC region in the organ of Corti of the apical turn of (A) wild-type and (B) and BACE1−/− mice stained with antibodies directed against presynaptic CtBP2 (red) and postsynaptic GluR2/3 (green). Dotted lines visualize individual IHCs in the region. C, CtBP2 and GluR2/3 puncta were quantified by blinded counting of immunopositive clusters from images as shown in (A+B) in BACE1+/+ (n = 41 cells of 3 mice) and BACE1−/− mice (n = 57 cells of 3 mice). There was no significant difference between genotypes. D, There was no obvious difference in the number of CtBP2-positive clusters in OHCs of BACE1+/+ (left) and BACE1−/− (right) mice. D, Representative confocal projections through the OHC region in the apical turn of BACE1+/+ (left) and BACE1−/− (right) mice stained with anti-CtBP2 antibodies (red) and DAPI (blue).

Journal: The Journal of Neuroscience

Article Title: β-Secretase BACE1 Is Required for Normal Cochlear Function

doi: 10.1523/JNEUROSCI.0028-19.2019

Figure Lengend Snippet: The architecture of ribbon synapses appears normal in BACE1−/− mice. A, B, Representative maximum intensity projections of confocal z-stacks from the IHC region in the organ of Corti of the apical turn of (A) wild-type and (B) and BACE1−/− mice stained with antibodies directed against presynaptic CtBP2 (red) and postsynaptic GluR2/3 (green). Dotted lines visualize individual IHCs in the region. C, CtBP2 and GluR2/3 puncta were quantified by blinded counting of immunopositive clusters from images as shown in (A+B) in BACE1+/+ (n = 41 cells of 3 mice) and BACE1−/− mice (n = 57 cells of 3 mice). There was no significant difference between genotypes. D, There was no obvious difference in the number of CtBP2-positive clusters in OHCs of BACE1+/+ (left) and BACE1−/− (right) mice. D, Representative confocal projections through the OHC region in the apical turn of BACE1+/+ (left) and BACE1−/− (right) mice stained with anti-CtBP2 antibodies (red) and DAPI (blue).

Article Snippet: Immunostaining was performed overnight at 4°C with the following primary antibodies (diluted in blocking solution): rabbit-anti-BACE1 (Abcam, ab108394; 1:50), guinea pig-anti-synapsin1,2 (Synaptic Systems, 106004; 1:500), rabbit-anti-KCNQ1 (K v 7.1) (Abcam, ab135737; 1:200), goat-anti-prestin (N-20; Santa Cruz Biotechnology, sc-22692; 1:400), rabbit-anti-KCNQ4 (K v 7.4) (H-130; Santa Cruz Biotechnology, sc-50417; 1:400), rabbit-anti-NF-200 (neurofilament heavy chain polypeptide, Sigma-Aldrich, N4142; 1:600) and chicken-anti-NF-H (neurofilament heavy chain polypeptide, Abcam, ab4680; 1:400), mouse-anti-CtBP2 (C-terminal binding protein; BD Bioscience, 612044; 1:200), rabbit-anti-GluR2/3 (Glutamate receptor subunits 2/3, Merck, ab1506; 1:200), rabbit-anti-PSD95 (postsynaptic density 95, Abcam, ab18258; 1:200), mouse-anti-MBP (myelin basic protein; F-6; Santa Cruz Biotechnology, sc-271524; 1:400), and rabbit-anti-B-FABP (brain-type fatty acid binding protein, Kurtz et al., 1994 , 1:1000).

Techniques: Staining

Disorganization of nerve fibers and ectopic overexpression of PSD95 in BACE1−/− mice. Auditory nerve fibers and the synaptic arrangement were analyzed in whole-mount organ of Corti preparations of the apical cochlear turn stained with primary antibodies targeted against neurofilament (NF-200 or NF-H, both are directed against neurofilament heavy chain polypeptide) and CtBP2 or PSD95, respectively. A, B, Auditory nerve fibers in the osseus spiral lamina (OSL) of BACE1−/− mice (in B; P48) showed more intense anti-NF-200 immunofluorescence, and appeared disorganized and swollen. In the OSL of BACE1−/− mice, we detected NF-200-positive patches with very high immunofluorescence (arrowheads in B) that were completely absent in wild-types. Dashed lines in A and B indicate the habenula perforata. Smaller images in A and B display magnifications of the OSL (top) and the IHC (bottom) region. C, D, Triple staining with antibodies directed against neurofilament (NF-H; blue), CtBP2 (presynaptic ribbon; red) and anti-PSD95 (postsynaptic density; green) showed that (C) in BACE1+/+ mice the presynaptic and postsynaptic terminals were well aligned in close proximity. D, In BACE1−/− mice a substantial number of PSD95-positive clusters was located far outside the synaptic region and not aligned at all with presynaptic anti-CtBP2 signals. Maximum intensity projections of confocal z-stacks shown in the acquisition plane (x–y, left) and in the orthogonal plane (z–y, right). E, Blinded counting of CtBP2- and PSD95-positive signals revealed significantly more PSD95 clusters in BACE1−/− than in BACE1+/+ mice (p < 0.001). In BACE1−/− mice, postsynaptic PSD95 clusters significantly outnumbered presynaptic CtBP2 clusters (p < 0.001). F, Schematic drawing summarizing our findings (neurofilament-positive fibers, blue; PSD95, orange; CtBP2, red; GluR2/3, green). (A–D) All images are representative confocal projections of the apical turn of the organ of Corti. Corresponding images (A,B and C,D) were taken in parallel under identical experimental conditions (See Materials and Methods). ***p<0.001.

Journal: The Journal of Neuroscience

Article Title: β-Secretase BACE1 Is Required for Normal Cochlear Function

doi: 10.1523/JNEUROSCI.0028-19.2019

Figure Lengend Snippet: Disorganization of nerve fibers and ectopic overexpression of PSD95 in BACE1−/− mice. Auditory nerve fibers and the synaptic arrangement were analyzed in whole-mount organ of Corti preparations of the apical cochlear turn stained with primary antibodies targeted against neurofilament (NF-200 or NF-H, both are directed against neurofilament heavy chain polypeptide) and CtBP2 or PSD95, respectively. A, B, Auditory nerve fibers in the osseus spiral lamina (OSL) of BACE1−/− mice (in B; P48) showed more intense anti-NF-200 immunofluorescence, and appeared disorganized and swollen. In the OSL of BACE1−/− mice, we detected NF-200-positive patches with very high immunofluorescence (arrowheads in B) that were completely absent in wild-types. Dashed lines in A and B indicate the habenula perforata. Smaller images in A and B display magnifications of the OSL (top) and the IHC (bottom) region. C, D, Triple staining with antibodies directed against neurofilament (NF-H; blue), CtBP2 (presynaptic ribbon; red) and anti-PSD95 (postsynaptic density; green) showed that (C) in BACE1+/+ mice the presynaptic and postsynaptic terminals were well aligned in close proximity. D, In BACE1−/− mice a substantial number of PSD95-positive clusters was located far outside the synaptic region and not aligned at all with presynaptic anti-CtBP2 signals. Maximum intensity projections of confocal z-stacks shown in the acquisition plane (x–y, left) and in the orthogonal plane (z–y, right). E, Blinded counting of CtBP2- and PSD95-positive signals revealed significantly more PSD95 clusters in BACE1−/− than in BACE1+/+ mice (p < 0.001). In BACE1−/− mice, postsynaptic PSD95 clusters significantly outnumbered presynaptic CtBP2 clusters (p < 0.001). F, Schematic drawing summarizing our findings (neurofilament-positive fibers, blue; PSD95, orange; CtBP2, red; GluR2/3, green). (A–D) All images are representative confocal projections of the apical turn of the organ of Corti. Corresponding images (A,B and C,D) were taken in parallel under identical experimental conditions (See Materials and Methods). ***p<0.001.

Article Snippet: Immunostaining was performed overnight at 4°C with the following primary antibodies (diluted in blocking solution): rabbit-anti-BACE1 (Abcam, ab108394; 1:50), guinea pig-anti-synapsin1,2 (Synaptic Systems, 106004; 1:500), rabbit-anti-KCNQ1 (K v 7.1) (Abcam, ab135737; 1:200), goat-anti-prestin (N-20; Santa Cruz Biotechnology, sc-22692; 1:400), rabbit-anti-KCNQ4 (K v 7.4) (H-130; Santa Cruz Biotechnology, sc-50417; 1:400), rabbit-anti-NF-200 (neurofilament heavy chain polypeptide, Sigma-Aldrich, N4142; 1:600) and chicken-anti-NF-H (neurofilament heavy chain polypeptide, Abcam, ab4680; 1:400), mouse-anti-CtBP2 (C-terminal binding protein; BD Bioscience, 612044; 1:200), rabbit-anti-GluR2/3 (Glutamate receptor subunits 2/3, Merck, ab1506; 1:200), rabbit-anti-PSD95 (postsynaptic density 95, Abcam, ab18258; 1:200), mouse-anti-MBP (myelin basic protein; F-6; Santa Cruz Biotechnology, sc-271524; 1:400), and rabbit-anti-B-FABP (brain-type fatty acid binding protein, Kurtz et al., 1994 , 1:1000).

Techniques: Over Expression, Staining, Immunofluorescence

FGF22 depletion reduces ribbon synapse number in vivo. AAV-shFGF22 and AAV-FGF22 were administrated to mouse cochlea to deplete or overexpress FGF22 in hair cells, respectively. Co-administration of AAV-shMEF2D with AAV-shFGF22 was also performed to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. Similarly, co-administration of AAV-MEF2D with AAV-FGF22 was performed, also to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. ( A , B ) TUNEL staining was performed on cochlea, showing by representative images ( A ), and by quantification ( B ). ( C , D ) The ribbon synapses were determined by co-staining for CtBP2 (in green) and GluR2&3 (in red). Cochlear ribbon synapse number was assessed, shown by representative images ( C ), and by quantification ( D ). *p<0.05. NS: non-significant. N=5. Scale bar is 20μm.

Journal: Aging (Albany NY)

Article Title: FGF22 promotes generation of ribbon synapses through downregulating MEF2D

doi: 10.18632/aging.103042

Figure Lengend Snippet: FGF22 depletion reduces ribbon synapse number in vivo. AAV-shFGF22 and AAV-FGF22 were administrated to mouse cochlea to deplete or overexpress FGF22 in hair cells, respectively. Co-administration of AAV-shMEF2D with AAV-shFGF22 was also performed to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. Similarly, co-administration of AAV-MEF2D with AAV-FGF22 was performed, also to assess the regulatory relationship between FGF22 and MEF2D in hair cells and ribbon synapses. ( A , B ) TUNEL staining was performed on cochlea, showing by representative images ( A ), and by quantification ( B ). ( C , D ) The ribbon synapses were determined by co-staining for CtBP2 (in green) and GluR2&3 (in red). Cochlear ribbon synapse number was assessed, shown by representative images ( C ), and by quantification ( D ). *p<0.05. NS: non-significant. N=5. Scale bar is 20μm.

Article Snippet: Primary antibodies are rabbit anti-CtBP2 polyclonal antibody (ab128871, 1:100, Abcam, Cambridge, MA, USA), rabbit anti-GluR2&3 polyclonal antibody (2mg/mL, Fisher Scientific, CA, USA) and rabbit anti-Myosin7a (1:200, ab3481, Abcam).

Techniques: In Vivo, TUNEL Assay, Staining